Coding

Part:BBa_I712002:Design

Designed by: Andrej Ondracka   Group: iGEM07_Ljubljana   (2007-10-21)

CCR5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 183


Design Notes

The part is cloned into BBa_J52017, a vector with eukaryontic terminator. PstI and EcoRI restriction sites inside CCR5 gene were mutated. The gene does NOT contain stop codon.


Source

CCR5 gene was obtained from OriGene, catalog No. SC110858.

References